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UV-. VIS spectrophotometry concerns the UV range covering of 200-380 nm general applications, but can be used for requirement for manual calculation. Mar 13, 2018 - Basic UV-Vis Theory, Concepts and Applications. Page 1 of 28. Introduction. Ultraviolet and visible spectrometers have been in general use for the last 35 years 4 Analytical Applications of UV-VIS Spectroscopy. For about 130 years, the Bouguer-Lambert-Beer law has been used as the quantitative basis of absorption Oct 26, 2006 - UV-visible spectroscopy. These provide valuable insight into the uses and limitations of this technique for chemical analysis. The primary applications of UV- tion of light in the visible region of the electromagnetic spectrum (400 ? 800 nm) and use absorption spectra to determine the pKa value of an indicator. Light in the UV-VIS part of the spectrum is used to promote electrons from the ground state to various excited states. • The particular frequencies at which light. Light in the UV-VIS part of the spectrum is used to promote electrons from the ground state to various excited states. • The particular frequencies at which light. Jul 19, 2016 -