Topo blunt end cloning kit manuals

zero blunt addgenetopo cloning
thermo zero blunt topo
zero blunt topo pcr cloning kit for sequencing
zero blunt™ topo™ pcr cloning kit
zero blunt topo pcr cloning kit with one shot top10 chemically competent e coli cells
zero blunt™ topo™ pcr cloning kit, without competent cells
zero blunt pcr cloning kit

At the moment I am trying to ligate a (pretty large) 3,8kb fragment (Gel-extraction purified in high yields) into a Zero Blunt TOPO vector using its original protocol. Zero Blunt TOPO PCR Cloning Kit provides a highly efficient, 5-minute, one-step Product overview. Manuals. MSDS. COA. FAQs. Citations & references. 6 Apr 2006 User Manual. pENTR. ™. Directional TOPO. ®. Cloning. Kits. Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry The TOPO TA Cloning® Kits for Sequencing are shipped on dry ice. Each kit contains a box . for your primers and template and be sure to end with a 7 to 30 minute .. plasmid DNA for automated or manual sequencing, we recommend the.14 Feb 2014 Zero Blunt® TOPO® PCR Cloning Kits are shipped on dry ice. Kits supplied with K2800-02, refer to the manual supplied with the miniprep kit. TOPO® TA Cloning® Kits supplied with the PureLink® Quick Plasmid Miniprep. Kit (Cat. nos. . K4510-02 and K4500-02 refer to the manual supplied with the miniprep kit. adds a single deoxyadenosine (A) to the 3? ends of PCR products. Whether you're performing general subcloning, sequencing, TA or high-fidelity blunt-end cloning, long-fragment cloning, expression-vector cloning, Zero Blunt TOPO PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading Follow these instructions to TOPO® Clone your blunt-end PCR product into pCR™-Blunt II-TOPO® and transform the reaction into chemically competent E. coli The pCR Blunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum SuperFi