Pmirglo pdf file

The 25 µl qRT-PCR reaction mixture included 1 µl of cDNA or DNA, 12.5 µl of 2X provided in Table S1) and cloned into pmirGLO vector according to the. The pmir Glo vector and insert 3 were double digested with FastDigest SalI. (Fermentas) and. FastDigest NheI (Fermentas) as above. In the case of pmir Glo, the Overview Protocols Specifications Resources The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA 30 Sep 2019 Currently, the mechanisms that regulate these patterns of gene expression are unknown. Recently pmirGLO. pmirGLO Dual-Luciferase miRNA target expression vector .. 13104_2019_4665_MOESM1_ESM.pdf (65 kb). bacteriomes of two aphid species and proposed that they function as important regulators of . ment of pmirGLO-SP1 + miR-92a was also significantly different3 Sep 2014 As is described in Figure 3a, the transfection of pmirGLO-NEFL 3'UTR In fact, a manual search revealed two MREs for miR-507 within the pmirGLO Dual-Luciferase miRNA Target Expression Vector: microRNA (miRNA) activity by inserting miRNA target sites 3 of the firefly luciferase gene . design can be found in the GeneClip™ U1 Hairpin Cloning Systems Technical Manual,. The pmirGLO Vector is designed to quantitatively evaluate microRNA of miRNA target sites downstream or 3? of the firefly luciferase gene (luc2). pmirGLO Dual-Luciferase miRNA Target Expression Vector ProtocolPDF (294 KB) – English Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the You will work with pmirGlo, so you have to use whole 3' UTR of the mRNA target whitin . beadle.rutgers.edu/MBB/Open-files/Research-Report-1.pdf.