Lightcycler 480 probes master manual

Roche Diagnostics LIGHTCYCLER 480 PROBES MASTER LIGHTCYCLER 480 PROBES MASTER Roche Diagnostics LIGHTCYCLER 480 PROBES MASTER . Manufacturer: Roche Diagnostics 04887301001 LightCycler 480 Probes Master - 10 x 5 ml (2x conc.) (approx. 10 x 500 reactionsof 20 uL final reaction volume) RNA LightCycler® 480 RNA Master Hydrolysis Probes Hydrolysis probes, UPL probes One-Step qRT-PCR Table 2: Application areas of the LightCycler® 480 Reagents. All LightCycler® 480 Reagents prevent carryover contamination by employing dUTP for UNG (uracil-DNA-glycosylase)-mediated decontamination. Extended storage conditions enable storage at TaqMan®?Fast Advanced Master Mix VS LightCycler™Probes Master TaqMan Fast Adv. and LC 480 Probes Master Comparison On the LightCycler™480 System II For Research Use Only. Not for use in diagnostic procedures. How LightCycler Probes Work. A LightCycler Probe system consists of a pair of single-stranded fluorescent-labeled oligonucleotides. Oligo Probe 1 is labeled at the 3' end with a donor reporter and Oligo Probe 2 is labeled at its 5' end with one of a few available acceptor reporters. LightCycler ® 480 Multiwell Plates 384 are full-skirted, white polypropylene reaction devices supplied with a barcoded label on each side of the plate. Their dimensions (inner/outer well geometry) and material are designed to achieve optimal results using the LightCycler ® 480 Instrument, 384-well, for reaction volumes from 5 to 20 ?l. lifescience.roche.com For general laboratory use. R LightCycler® EvoScript RNA Probes Master y Version: 02 Content version: May 2017 Easy-to-use reaction mix for one-step RT-qPCR using the LightCyler® 480 or the LightCycler® 96 Real-Time PCR Systems Cat. No. 07 800 029 001 1 kit 200 reactions of 20 µl final volume each Quantitative PCR analysis was performed using LightCycler 480 SYBR Green I Master (Roche diagnostics, Mannheim, Germany). ??actin was used as an internal control gene to analyze the mRNA expression. .. The