Results: FISH allowed the detection of H. pylori in 20 out of 26 clinical samples and 12 samples were positive in wastewater. The 35% of the positive biopsies showed the presence of clarithromycin resistant strains and 16.6% of the positive wastewater samples yielded resistance genotype to this macrolide. The cells were sonicated (Branson Sonifier 350, Danbury, USA) on ice for 30 min at an amplitude of 15% with 1.5 s pulse and 3 s interval. The sonicate was centrifuged (Eppendorf centrifuge 5810r, Hamburg, Germany) at 4 °C, 18,500 ? g for 30 min and the supernatant was transferred to a clean centrifuge tube. NaCl was added to the supernatant This is a good working Eppendorf 5415R refrigerated centrifuge with F45-24-11 rotor and lid. Eppendorf 5415R Refrigerated Micro-Centrifuge, 13,200 RPM Max, 120V/50Hz. F45-24-11 Eppendorf Fixed Angle R Description Choose either the 5702 model - a base, non-heated centrifuge for standard applications, the 5702 R that is a refrigerated model for heat-sensitive samples, or the heated and refrigerated 5702 RH model for centrifugation under biological conditions to improve cell viability for more accurate results in downstream application like cell cultivation. In many cases, a positive MAC-ELISA with an acute serum sample precludes the need for testing of a convalescent serum sample. Early in infection, IgM antibody is more serocomplex specific, while later in the infection, IgG antibody is more serocomplex cross-reactive. Centrifugation and Rotor Service Get your service for your samples to spin safely! Learn more about our plans. Eppendorf Training Become an expert with our training program. Are you Prepared? Inform yourself about safe operation of centrifuges. Eppendorf Centrifuge 5415 R with Error. This feature is not available right now. Please try again later. the back of the centrifuge. The centrifuge must operate on a flat and stable work surface to ensure proper and safe operation. Note: Save all packaging f
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